Protein Modeling C

[JoeyC]

List the groups of amino acids, and if they are polar, nonpolar, charged, or uncharged.

[whythelongface]

Aliphatic
Glycine
Valine
Alanine
Leucine
Isoleucine
Proline
Methionine

Aromatic
Phenylalanine - nonpolar
Tyrosine - polar
Tryptophan - nonpolar

Polar positive - basic at pH 7
Lysine
Arginine
Histidine

Polar negative - acidic at pH 7
Glutamate
Aspartate

Polar neutral
Cysteine
Threonine
Serine
Asparagine
Glutamine

Aliphatic R-groups are non-polar, lipophillic, and hydrophobic, the polarity of aromatic R-groups is found by counting the number of polar groups attached to the rings, polar positives are basic at physiological pH because they are protonated (and polar negatives are acidic at physiological pH because they are deprotonated). Polar neutrals are neither strongly acidic nor basic. Polar R-groups are hydrophillic and lipophobic. I admit that had to consult Lehninger's for some of these because I hadn't memorized every single one of them. Technically I'm not a competitor anymore but it's been over a month and I guess I wanted to revive this thread. Plus, maybe it will help me write tests!

[platypusomelette]

Anyone wanna post new questions?

[naddruf]

Okay.
What signals cause cas9 to bind to viral DNA?

[platypusomelette]

Okay.
What signals cause cas9 to bind to viral DNA?

Cas9 first recognizes the PAM sequence in foreign viral DNA, then if that DNA matches the sequence of the crRNA in cas9 it will be cut. I'll ask some more questions, you guys dont have to answer all of them

1. The RuvC subunit of cas9 does what?
2. Where on a ramachandran plot would you find angles representing beta sheets? A. middle left, B. middle right, C. top left, or D. top left
3. A gRNA and crRNA combined into a single strand is called ______.
4. Are all naturally occurring amino acids an L isomer or a D isomer?
5. Name one amino acid that contains a ring.
6. What is meant by a "salt bridge"?

[BrownieInMotion]

1. The RuvC subunit of cas9 does what?
2. Where on a ramachandran plot would you find angles representing beta sheets? A. middle left, B. middle right, C. top left, or D. top left
3. A gRNA and crRNA combined into a single strand is called ______.
4. Are all naturally occurring amino acids an L isomer or a D isomer?
5. Name one amino acid that contains a ring.
6. What is meant by a "salt bridge"?

I just discovered question marathons today owo. I don't know CRISPR stuff so I'll skip those heh.

My answers to 2, 4, 5, 6 (Click to Show Hidden Text)

2. Top left
4. L
5. Tyrosine has one
6. Combination of h-bonding and ionic bonding between two oppositely charged residues.

Let me know if these are right

[whythelongface]

1. The RuvC subunit of cas9 does what?
2. Where on a ramachandran plot would you find angles representing beta sheets? A. middle left, B. middle right, C. top left, or D. top left
3. A gRNA and crRNA combined into a single strand is called ______.
4. Are all naturally occurring amino acids an L isomer or a D isomer?
5. Name one amino acid that contains a ring.
6. What is meant by a "salt bridge"?

I just discovered question marathons today owo. I don't know CRISPR stuff so I'll skip those heh.

My answers to 2, 4, 5, 6 (Click to Show Hidden Text)

2. Top left
4. L
5. Tyrosine has one
6. Combination of h-bonding and ionic bonding between two oppositely charged residues.

Let me know if these are right

3. Is tracrRNA I think

[platypusomelette]

I just discovered question marathons today owo. I don't know CRISPR stuff so I'll skip those heh.

My answers to 2, 4, 5, 6 (Click to Show Hidden Text)

2. Top left
4. L
5. Tyrosine has one
6. Combination of h-bonding and ionic bonding between two oppositely charged residues.

Let me know if these are right

3. Is tracrRNA I think

Oh god sorry guys I just realized what a train wreck my questions were lol. For 3 I meant to say a combination of gRNA and tracrRNA which would be sgRNA 1. RuvC is a catalytic site that cuts the DNA
2. Correct
3. sgRNA
4. Correct
5. Correct
6. Correct Okk your turns